Discovery of a Novel β-xylosidase with Xylanase Activity and Its Application in the Production of Xylitol from Corncob Xylan

Although β-xylosidases with xylanase activity are preferential for the hydrolysis of xylan and production xylitol, reports on their use scarce. In this study, a multifunctional β-xylosidase (XYL4) was identified. addition to activity, XYL4 also exhibited low α-arabinosidase activity. The enzyme able hydrolyze bagasse xylan, oat spelt birchwood beechwood corncob showed highest xylan. Structural modeling analysis indicated that had an additional PA14 domain, which may play key role in binding substrates. Moreover, used produce xylose. When enzymatic whole-cell catalysis were 100 g/L xylose yields 60.26% 35.85%, respectively. Then, Candida tropicalis inoculated above hydrolysates fermentation xylitol. Using catalysis, xylitol 77.56% 73.67% obtained by C. after optimization fermentation,